Knockdown of survivin expression by siRNAs enhances chemosensitivity of prostate cancer cells and attenuates its tumorigenicity
1 Department of Clinical Laboratory, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China
2 Department of Medical Genetics and Developmental Biology, Fourth Military Medical University, Xi'an 710032, China
* Correspondence address. Tel: +86-29-84775959; Fax: +86-29-82527499; E-mail: haoxkg{at}fmmu.edu.cn
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Abstract |
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Survivin, a member of inhibitor of apoptosis family protein, has become an attractive therapeutic target in cancer due to its selective expression in tumor cells and its important roles for tumor cell viability. Here, we show that vector-based small interfering RNAs (siRNAs) silenced survivin expression in prostate cancer cells, resulting in significantly reduced cell proliferation and enhanced apoptosis, and increased the sensitivity of prostate cancer cells (PC-3) to the apoptosis-inducing agent, platinol. Furthermore, PC-3 cells transfected with the siRNA-expressing vector showed lower tumor formation in nude mice xenografts in vivo. These results demonstrated that inhibition of survivin expression by siRNA attenuated the malignant phenotypes of prostate cancer cells, and may provide a novel approach for gene therapy of androgen-independent prostate cancer.
Keywords RNA interference; survivin; prostate cancer; apoptosis; gene therapy
Received: August 16, 2008; Accepted: December 12, 2008
These authors contributed equally to this work.
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