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Acta Biochimica et Biophysica Sinica 2009 41(2):146-153; doi:10.1093/abbs/gmn017
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© The Author 2009. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

Effect of LDL concentration polarization on the uptake of LDL by human endothelial cells and smooth muscle cells co-cultured

Zufeng Ding, Yubo Fan and Xiaoyan Deng*

School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China

* Correspondence address. Tel: 86-10-82339742; Fax: 86-10-82339428; E-mail: dengxy1953{at}buaa.edu.cn


  Abstract

To substantiate our hypothesis that concentration polarization of low-density lipoprotein (LDL) plays an important role in the localization of atherogenesis, we investigated the effects of wall shear stress and water filtration rate (or perfusion pressure) on the luminal surface LDL concentration (cw) and the LDL uptake by human vascular endothelial cells and smooth muscle cells co-cultured on a permeable membrane using a parallel-plate flow chamber technique and a flow cytometry method. The results indicated that the uptake of fluorescent labeled LDL (DiI-LDL) by the co-cultured cells was positively correlated with cw in a non-linear fashion. When cw was low, the uptake increased very sharply with increasing cw. Then the increase became gradual and the uptake was seemingly leveled out when cw reached beyond 160 µg/ml. The present study therefore has provided further experimental evidence that concentration polarization may occur in the arterial system and have a positive correlation with the uptake of LDLs by the arterial wall, which gives support to our hypothesis regarding the localization of atherogenesis.

Keywords    atherosclerosis; concentration polarization; shear stress; filtration rate

Received: September 21, 2008; Accepted: November 20, 2008
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