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Acta Biochimica et Biophysica Sinica 2009 41(2):123-130; doi:10.1093/abbs/gmn014
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© The Author 2009. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

Regulation of polyphenols accumulation by combined overexpression/silencing key enzymes of phyenylpropanoid pathway

Junli Chang{dagger}, Jie Luo{dagger} and Guangyuan He*

China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Key Laboratory of Molecular Biophysics, Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology (HUST), Wuhan 430074, China

* Correspondence address. Tel: +86-27-87792271; Fax: +86-27-87792272; E-mail: guangyuan.he{at}bbsrc.ac.uk; hegy{at}hust.edu.cn


  Abstract

There is a growing interest in the metabolic engineering of plant with increased desirable polyphenols such as chlorogenic acid (CGA) and rutin. In this study, the effects of overexpression of both phenylalanine ammonia lyase (AtPAL2), the first enzyme of the phenylpropanoid pathway, and hydroxycinnamoyl-CoA quinate:hydroxycinnamoyl transferase (NtHQT), the last enzyme of CGA biosynthesis, and the overexpression of AtPAL2 together with silencing of NtHQT were investigated in tobacco. Transgenic tobacco plants overexpressing AtPAL2 showed two and five times increases of CGA and rutin levels than the wild-type (WT) plants, respectively. Overexpression of NtHQT further increases the accumulation of CGA in the AtPAL2 plants to about three times than that of the WT level, while silencing of NtHQT in AtPAL2 plants results in ~12 times increase in rutin level than that of the WT plants. Simultaneous overexpression of phenylalanine ammonia lyase (PAL) and overexpression/silencing HQT could be used for the production of functional food with increased polyphenols.

Keywords    phenylalanine ammonia lyase; hydroxycinnamoyl-CoA quinate:hydroxycinnamoyl transferase; phenylpropanoid; metabolic engineering

Received: September 8, 2008; Accepted: November 19, 2008

{dagger} These authors contributed equally to this work


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