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Acta Biochimica et Biophysica Sinica Advance Access published online on November 10, 2009
Acta Biochimica et Biophysica Sinica, doi:10.1093/abbs/gmp099
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© The Author 2009. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

Antioxidant activity of mangostin in cell-free system and its effect on K562 leukemia cell line in photodynamic therapy

Dan Sun1, Sujuan Zhang1,*, Yongfeng Wei2 and Lingfang Yin3

1 Institute of Photonics and Phototechnology, Northwest University, Xi'an 710069, China
2 Department of Chemistry, Northwest University, Xi'an 710069, China
3 College of Life Sciences, Northwest University, Xi'an 710069, China

* Correspondence address. Tel/Fax: +86-29-88302351; E-mail: sujuan_zhang{at}yahoo.com.cn


  Abstract

Mangostin (MAG), a kind of xanthone widely used in diet and medicine, has antioxidant, anti-inflammatory, antimicrobial, and anticancer activities. On account of its antioxidant activity, MAG might protect cancer cells from free radical damage in photodynamic therapy (PDT) during which reactive oxygen species production was stimulated leading to irreversible tumor cell injury. In this study, the antioxidant activity of MAG was investigated and the influence of MAG on K562 cells in 5-aminolevulinic acid (ALA)-based PDT is demonstrated. The results showed that MAG could scavenge hydroxyl radical, superoxide anion, and hydrogen peroxide and inhibit the formation of malondialdehyde (MDA), but increase the amounts of singlet oxygen in cell-free systems. MAG inhibits cell proliferation and enhances cell apoptosis, lipid peroxidation, and DNA damage in ALA-PDT on K562 cells. NaN3, a singlet oxygen quencher, suppresses the MAG-induced cell apoptosis, lipid peroxidation, and DNA damage. In conclusion, MAG enhances the PDT-induced cytotoxicity in K562 cells and singlet oxygen was involved in this process. These results implied that the effect of antioxidants on PDT might be determined by its sensitization ability to singlet oxygen.

Keywords    mangostin; reactive oxygen species; photodynamic therapy; NaN3; leukemia K562

Received: June 22, 2009; Accepted: September 1, 2009
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