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Acta Biochimica et Biophysica Sinica Advance Access originally published online on August 1, 2009
Acta Biochimica et Biophysica Sinica 2009 41(9):800-807; doi:10.1093/abbs/gmp069
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© The Author 2009. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

The role of MAPK and FAS death receptor pathways in testicular germ cell apoptosis induced by lead

Shuying Dong1, Duoping Liang2,*, Na An1, Li Jia1, Yujuan Shan1, Chao Chen1, Kuo Sun1, Fei Niu1, Huiyan Li1 and Songbin Fu1

1 Institute of Public Health, Harbin Medical University, Harbin 150086, China
2 Hangzhou Dianzi University, Hangzhou 310000, China

* Correspondence address. Tel: +86-571-86878632; E-mail: liangduoping{at}hdu.edu.cn


  Abstract

The aim of the present study is to investigate gene expression involved in the signal pathway of MAPK and death signal receptor pathway of FAS in lead-induced apoptosis of testicular germ cells. First, cell viabilities were determined by MTT assay. Second, using single cell gel-electrophoresis test (comet assay) and TUNEL staining technique, apoptotic rate and cell apoptosis localization of testicular germ cells were measured in mice treated with 0.15%, 0.3%, and 0.6% lead, respectively. Third, the immunolocalization of K-ras, c-fos, Fas, and active caspase-3 proteins was determined by immunohistochemistry. Finally, changes in the translational levels of K-ras, c-fos, Fas, and active caspase-3 were further detected by western blot analysis. Our results showed that lead could significantly induce testicular germ cell apoptosis in a dose-dependent manner (P < 0.01). The mechanisms were closely related to the increased expressions of K-ras, c-fos, Fas, and active caspase-3 in apoptotic germ cells. In conclusion, K-ras/c-fos and Fas/caspase-3 death signaling receptor pathways were involved in the lead-induced apoptosis of the testicular germ cells in mice.

Keywords    lead acetate; testis; cell apoptosis; K-ras; c-fos; Fas; caspase-3

Received: April 1, 2009; Accepted: June 21, 2009
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