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Acta Biochimica et Biophysica Sinica Advance Access originally published online on August 6, 2009
Acta Biochimica et Biophysica Sinica 2009 41(9):754-762; doi:10.1093/abbs/gmp064
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© The Author 2009. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

Preparation of polyclonal antibody specific for NOR1 and detection of its expression pattern in human tissues and nasopharyngeal carcinoma

Bo Xiang1,{dagger}, Mei Yi2,{dagger}, Li Wang1,{dagger}, Wei Liu3,{dagger}, Wenling Zhang1, Jue Ouyang1, Ya Peng4, Wenjuan Li1, Ming Zhou1, Huaying Liu1, Minghua Wu1, Rong Wang1, Xiaoling Li1 and Guiyuan Li1,*

1 Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China
2 Department of Dermatology, Xiangya Hospital of Central South University, Changsha 410078, China
3 Department of Endocrinology, Second Xiangya Hospital, Central South University, Changsha 410011, China
4 Department of Gastrioenterology, Third Xiangya Hospital, Central South University, Changsha 410013, China

* Correspondence address. Tel: +86-731-4805383; Fax: +86-731-4805383. E-mail: ligy{at}xysm.net


  Abstract

Oxidored-nitro domain containing protein 1 (NOR1) gene is a novel nitroreductase gene first isolated from nasopharyngeal carcinoma (NPC). It plays an important role in the formation of chemical carcinogen and the carcinogenesis of NPC for its nitrosation function. Overexpression of the wild-type NOR1 gene in nasopharyngeal carcinoma cells is effective to inhibit cell growth and proliferation. In this study, for the first time, we generated a highly specific NOR1 antibody and analyzed NOR1 distribution in the human tissues and NPC biopsies. The results showed that NOR1 protein is predominantly expressed in human nasopharynx and tracheal tissues. Human heart, liver, spleen, stomach, colon, kidney, skeletal muscle, thymus, and pancreas are all deficient of NOR1 protein. More importantly, we performed immunohistochemistry assay of NOR1 protein expression in the NPC tissues, and the result showed that NOR1 protein is frequently down-expressed in NPC. These data shed light on the selectivity of potential physiological functions of NOR1 and provides an indispensable reference to the carcinogenesis process of NPC and to identify or validate tissue-specific drug targets.

Keywords    nitroreductase; NOR1; antibody; tissue distribution; nasopharyngeal carcinoma

Received: March 6, 2009; Accepted: May 5, 2006

{dagger} These authors contributed equally to this work.


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