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Acta Biochimica et Biophysica Sinica Advance Access originally published online on August 5, 2009
Acta Biochimica et Biophysica Sinica 2009 41(9):737-744; doi:10.1093/abbs/gmp062
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© The Author 2009. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

Effects of a chemically derived homo zwitterionic polysaccharide on immune activation in mice

Chun Meng, Xu Peng, Xian'ai Shi, Hang Wang and Yanghao Guo*

College of Biological Science and Biotechnology, Fuzhou University, Fuzhou 350108, China

* Correspondence address. Tel: +86-591-22866379; Fax: +86-591-22866379; E-mail: bioeng{at}fzu.edu.cn


  Abstract

In this study, a chemically modified homo zwitterionic polysaccharide (ZPS), sulfated chitosan, was used to examine its effects on murine immune response. The results showed that homoZPS could markedly promote the proliferation of both splenic T/B cells and adhesive cells. In particular, flow cytometry assay demonstrated that the sulfated chitosan could non-specifically activate CD3+ and CD8+ T cells proliferation in vitro. The effectiveness of sulfated chitosan as adjuvant was tested using bovine serum albumin (BSA) and diphtheria toxin (DT) as antigens and compared with that of aluminum hydroxide. The levels of specific antibodies to BSA and DT significantly increased after homoZPS vaccination. Both homoZPS and aluminum hydroxide adjuvants could protect mice against the attack of DT from edemas of spleen and tail. The present findings demonstrated the chemically derived homoZPS could be a potential candidate in the development of T-lymphocyte dependent vaccine adjuvants.

Keywords    chemically derived homoZPS; sulfated chitosan; immune response; splenic cells; adjuvant

Received: February 9, 2009; Accepted: April 29, 2009
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