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Acta Biochimica et Biophysica Sinica Advance Access originally published online on July 23, 2009
Acta Biochimica et Biophysica Sinica 2009 41(9):719-730; doi:10.1093/abbs/gmp060
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© The Author 2009. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

Post-transcriptional regulation of NifA expression by Hfq and RNase E complex in Rhizobium leguminosarum bv. viciae

Yinghua Zhang1,2 and Guofan Hong1,*

1 State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
2 Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China

* Correspondence address. Tel: +86-21-54921223; Fax: +86-21-54921011; E-mail: gfhong{at}sibs.ac.cn


  Abstract

NifA is the general transcriptional activator of nitrogen fixation genes in diazotrophic bacteria. In Rhizobium leguminosarum bv. viciae strain 8401/pRL1JI, the NifA gene is part of a gene cluster (fixABCXNifAB). In this study, results showed that in R. leguminosarum bv. viciae 8401/pRL1JI, host factor required (Hfq), and RNase E were involved in the post-transcriptional regulation of NifA expression. It was found that Hfq-dependent RNase E cleavage of NifA mRNA was essential for NifA translation. The cleavage site is located at 32 nucleotides upstream of the NifA translational start codon. A possible explanation based on predicted RNA secondary structure of the NifA 5'-untranslated region was that the cleavage made ribosome-binding sites accessible for translation.

Keywords    Hfq; RNase E; NifA; Rhizobium leguminosarum bv. viciae

Received: March 3, 2009; Accepted: April 21, 2009
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