Skip Navigation


Acta Biochimica et Biophysica Sinica Advance Access originally published online on March 24, 2009
Acta Biochimica et Biophysica Sinica 2009 41(4):335-340; doi:10.1093/abbs/gmp022
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
41/4/335    most recent
gmp022v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Wang, C.
Right arrow Articles by Ding, H.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Wang, C.
Right arrow Articles by Ding, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

In vitro selection of high-affinity DNA aptamers for streptavidin

Chenglong Wang1,2,*, Guang Yang2, Zhaofeng Luo3 and Hongmei Ding2

1 Department of Stomatology, General Hospital of PLA, Beijing 100853, China
2 Beijing Institute of Basic Medical Sciences, Beijing 100850, China
3 Center of Bioscience, University of Science and Technology of China, Hefei 230027, China

* Correspondence address. Tel: +86-10-66937964; Fax: +86-10-66936254; E-mail: wangchenglong9{at}hotmail.com


  Abstract

In this study, we developed a systematic evolution of ligands by exponential enrichment (SELEX) method using a combination of magnetic beads immobilization and flow cytometric measurement. As an example, the selection of streptavidin-specific aptamers was performed. In this protocol, the conventional SELEX procedure was optimized, first using magnetic beads for target immobilization to facilitate highly efficient separation of the binding single-stranded DNA (ssDNA) aptamers from the unbound ssDNAs, and second using flow cytometry and fluorescein labeling to monitor the enrichment. The sensitivity of flow cytometry was adequate for ssDNA quantification during the SELEX procedures. The streptavidin-specific aptamers obtained in this work can be used as tools for characterization of the occupancy of streptavidin-modified surfaces with biotinylated target molecules. The method described in the study is also generally applicable to target molecules other than streptavidin.

Keywords    magnetic beads; flow cytometry; SELEX; aptamers; streptavidin

Received: November 15, 2008; Accepted: February 16, 2009
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.