Skip Navigation


Acta Biochimica et Biophysica Sinica Advance Access originally published online on October 8, 2009
Acta Biochimica et Biophysica Sinica 2009 41(11):929-937; doi:10.1093/abbs/gmp088
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
41/11/929    most recent
gmp088v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Li, X.
Right arrow Articles by Dong, X.
PubMed
Right arrow Articles by Li, X.
Right arrow Articles by Dong, X.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

The octarepeat region of hamster PrP (PrP51–91) enhances the formation of microtubule and antagonize Cu2+-induced microtubule-disrupting activity

Xiaoli Li{dagger}, Chenfang Dong{dagger}, Song Shi, Guirong Wang, Yuan Li, Xin Wang, Qi Shi, Chan Tian, Ruimin Zhou, Chen Gao and Xiaoping Dong*

State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China

* Correspondence address. Tel: +86-10-83534616; Fax: +86-10-63532053; E-mail: dongxp238{at}sina.com


  Abstract

Prion protein (PrP) is considered to associate with microtubule and its major component, tubulin. In the present study, octarepeat region of PrP (PrP51–91) was expressed in prokaryotic-expressing system. Using GST pull-down assay and co-immunoprecipitation, the molecular interaction between PrP51–91 and tubulin was observed. Our data also demonstrated that PrP51–91 could efficiently stimulate microtubule assembly in vitro, indicating a potential effect of PrP on microtubule dynamics. Moreover, PrP51–91 was confirmed to be able to antagonize Cu2+-induced microtubule-disrupting activity in vivo, partially protecting against Cu2+ intoxication to culture cells and stabilize cellular microtubule structure. The association of the octarepeat region of PrP with tubulin may further provide insight into the biological function of PrP in the neurons.

Keywords    prion protein; octarepeat region; tubulin; molecular interaction; microtubule dynamics

Received: March 26, 2009; Accepted: July 20, 2009

{dagger} These authors contributed equally to this work.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.