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Acta Biochimica et Biophysica Sinica Advance Access originally published online on October 2, 2009
Acta Biochimica et Biophysica Sinica 2009 41(11):922-928; doi:10.1093/abbs/gmp081
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© The Author 2009. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

Phenotypic inheritance induced by hairpin RNA in Drosophila

Huaguang Li1,2,* and Yi Lu1

1 CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Shanghai 200031, China
2 Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China

* Correspondence address. Tel: +86-21-54921139; Fax: +86-21-54921126; E-mail: hgli{at}sibs.ac.cn


  Abstract

Phenotypic inheritance induced by RNA has been documented in mouse and Caenorhabditis elegans. Here we report a similar inheritance in Drosophila. Mutant phenotypes of eye defects and antenna duplication generated from the crossing of one RNA interference (RNAi) transgenic line harboring one hairpin RNA transgene with a GAL4 driver line were inherited independently of the GAL4 driver. Hairpin RNA injection experiments demonstrated that the hairpin RNA could induce heritable mutant-like phenotypes on the eye and antenna. The penetrance of mutant phenotypes was reduced when the mutants were crossed to ago1 and piwi mutants. Our data suggest that hairpin RNA can induce phenotypic inheritance in Drosophila.

Keywords    phenotypic inheritance; Drosophila; hairpin RNA

Received: April 13, 2009; Accepted: July 14, 2009
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