RanBPM is an acetylcholinesterase-interacting protein that translocates into the nucleus during apoptosis
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
* Correspondence address. Tel: +86-21-54921403; Fax: +86-21-54921403; E-mail: xjzhang{at}sibs.ac.cn
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Abstract |
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Acetylcholinesterase (AChE) expression may be induced during apoptosis in various cell types. Here, we used the C-terminal of AChE to screen the human fetal brain library and found that it interacted with Ran-binding protein in the microtubule-organizing center (RanBPM). This interaction was further confirmed by coimmunoprecipitation analysis. In HEK293T cells, RanBPM and AChE were heterogeneously expressed in the cisplatin-untreated cytoplasmic extracts and in the cisplatin-treated cytoplasmic or nuclear extracts. Our previous studies performed using morphologic methods have shown that AChE translocates from the cytoplasm to the nucleus during apoptosis. Taken together, these results suggest that RanBPM is an AChE-interacting protein that is translocated from the cytoplasm into the nucleus during apoptosis, similar to the translocation observed in case of AChE.
Keywords AChE; RanBPM; yeast two-hybrid; cisplatin; apoptosis
Received: April 21, 2009; Accepted: June 21, 2009
These authors contributed equally to this work.