Skip Navigation


Acta Biochimica et Biophysica Sinica Advance Access originally published online on September 8, 2009
Acta Biochimica et Biophysica Sinica 2009 41(10):831-838; doi:10.1093/abbs/gmp072
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
41/10/831    most recent
gmp072v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Gu, J.
Right arrow Articles by Wei, W.
PubMed
Right arrow Articles by Gu, J.
Right arrow Articles by Wei, W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

Human Elongator complex is involved in cell cycle and suppresses cell growth in 293T human embryonic kidney cells

Junxia Gu1,2, Dongmei Sun1, Qiping Zheng3, Xiaochun Wang1, Huicui Yang1, Jingcheng Miao1, Jingting Jiang4,* and Wenxiang Wei1,*

1 Department of Cellular and Molecular Biology, School of Medicine, Suzhou University, Suzhou 215123, China
2 Department of Hematology and Hematological Laboratory Science, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013, China
3 Department of Anatomy and Cell Biology, Rush University Medical Center, Chicago, IL 60612, USA
4 Department of Tumor Biotherapy, Third Affiliated Hospital of Suzhou University, Changzhou 213003, China

* Correspondence address. Tel: +86-512-65880107; Fax: +86-512-65880103; E-mail: wenxiangw{at}suda.edu.cn (W.W.); Tel: +86-519-86180978; Fax: +86-519-86621235; E-mail: jjtnew{at}163.com (J.J.)


  Abstract

Elongator complex has been associated with hyperphosphorylated RNA polymerase II and is known to play critical roles in transcriptional elongation, as well as in tRNA modification and exocytosis. However, the specific mechanism of how human Elongator complex regulates cell growth and cell cycle remains unclear. To investigate the composition of human Elongator complex and its effects on cell growth, 293T cells were established that stably overexpressed Flag-Elp3 and Flag-Elp4. By using anti-Flag M2 antibody-bound resin, a core Elongator complex was purified from cells that stably overexpressed Flag-Elp3. No Elongator complex was purified from cells stably transfected with pFlagCMV4-Elp4. Interestingly, the cell growth was inhibited in 293T cells transfected with pFlagCMV4-Elp3. Flow cytometry analysis showed that most of the cells stably overexpressing Flag-Elp3 were found in G1 stage, indicating a role of the core Elongator in the G1 checkpoint for the regulation of cell cycle. We observed increased basal transcription and remarkably enhanced transcription stimulated by VP16 in 293T cells overexpressing Flag-Elp3. The transcription could also be synergistically activated by overexpressing both Elp3 and Elp4. Taken together, our results suggested that the core Elongator complex formed by Elp1, Elp2, and Elp3 was rather stable, whereas Elp4, Elp5, and Elp6 might loosely contact and work together with the core Elongator to regulate cell functions.

Keywords    Elongator; Elp3; Elp4; cell cycle

Received: April 28, 2009; Accepted: June 29, 2009
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.