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Acta Biochimica et Biophysica Sinica Advance Access originally published online on September 8, 2009
Acta Biochimica et Biophysica Sinica 2009 41(10):822-830; doi:10.1093/abbs/gmp073
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© The Author 2009. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

A small functional intramolecular region of NodD was identified by mutation

Bihe Hou, Fengqing Li, Xiao'er Yang and Guofan Hong*

State key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China

* Correspondence address. Tel: +86-21-54921223; Fax: +86-21-54921011; E-mail: gfhong{at}sibs.ac.cn


  Abstract

In Rhizobium leguminosarum bv. viciae, NodD, as a member of the LysR-type transcriptional regulators (LTTRs), exerts auto-regulation and activates transcription of other nod genes in the presence of naringenin. LTTRs were typically composed of N-terminal DNA-binding domain and C-terminal regulatory domain. In this study, by systematic insertion mutation, a region of 12 amino acids in length of NodD was identified as functional domain. Insertion mutants in this region appeared to acquire the ability of constitutively activating nodA gene and retained their auto-regulation properties. This identified region was shown to be a hinge of NodD as revealed through the model built using Swiss-PDB Viewer software. It is the first time to report that as a member of LysR family, NodD has been shown to contain a short intramolecular domain that influences its performance.

Keywords    NodD; insertion mutation; hinge region

Received: March 24, 2009; Accepted: May 12, 2009
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B. Hou, F. Li, X. Yang, and G. Hong
The properties of NodD were affected by mere variation in length within its hinge region
Acta Biochim Biophys Sin, November 1, 2009; 41(11): 963 - 971.
[Abstract] [Full Text] [PDF]


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