Skip Navigation


Acta Biochimica et Biophysica Sinica Advance Access originally published online on September 6, 2009
Acta Biochimica et Biophysica Sinica 2009 41(10):816-821; doi:10.1093/abbs/gmp074
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
41/10/816    most recent
gmp074v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Du, J.
Right arrow Articles by Shen, J.
PubMed
Right arrow Articles by Du, J.
Right arrow Articles by Shen, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

Identification of FANCA as a protein interacting with centromere-associated protein E

Jian Du1,*,{dagger}, Lijian Chen2,{dagger} and Jilong Shen3

1 Department of Biochemistry and Molecular Biology, Anhui Medical University, Hefei 230032, China
2 Department of Anaesthesiology, the First Affiliated Hospital of Anhui Medical University, Hefei 230032, China
3 Department of Microbiology and Parasitology, Provincial Key Laboratory of Genomic Research, Anhui Medical University, Hefei 230032, China

* Correspondence address. Tel: +86-551-5161131; Fax: +86-551-5161131; E-mail: dudu{at}mail.ustc.edu.cn


  Abstract

This study sought to isolate and identify proteins that interact with centromere-associated protein E (CENP-E), provide new clues for exploring the function of CENP-E in cell cycle control and the pathogenesis of tumor. Yeast two-hybrid screen and regular molecular biologic techniques were undertaken to screen human HeLa cDNA library with the kinetochore binding domain of CENP-E. The bait from the C-terminus of CENP-E was created by subcloning methods to find out optimal candidate proteins that interact with the kinetochore binding domain of CENP-E. Eight novel CENP-E interacting proteins including Homo sapiens Fanconi anemia complementation group A (FANCA) were obtained. In yeast two-hybrid assay, the N-terminal 260 amino acids of FANCA were found to be necessary and sufficient for the interaction with the C-terminus of CENP-E. The interaction was confirmed by in vitro glutathione S-transferase pull-down assay and in vivo co-immunoprecipitation assay. Our finding of the interaction of CENP-E with FANCA demonstrates that CENP-E and FANCA may play important roles in the functional regulation of the mitotic checkpoint signal pathway.

Keywords    Fanconi anemia complementation group A (FANCA); centromere-associated protein E; yeast two-hybrid; protein interaction

Received: March 21, 2009; Accepted: June 8, 2009

{dagger} These authors contributed equally to this work.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.