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Acta Biochimica et Biophysica Sinica 2009 41(1):79-85; doi:10.1093/abbs/gmn009
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© The Author 2009. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

Cloning, expression, and protective immunity in mice of a gene encoding the diagnostic antigen P-29 of Echinococcus granulosus

Zhiyun Shi1, Yana Wang1, Zongji Li1, Zhaoyu Li2, Yang Bo2, Rui Ma1 and Wei Zhao1,*

1 Department of Medical Genetics and Cell Biology, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, 750004, China
2 Department of Liver and Gallbladder Surgery, The Affiliated Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, 750004, China

* Corresponding author: Tel, 86-951-6980125; Fax, 86-951-6980125; E-mail, zw-6915{at}163.com


  Abstract

Taeniid tapeworm Echinococcus granulosus is the causative agent of Echinococcosis, an important zoonosis with worldwide distribution. In this study, a diagnostic antigen P-29 was cloned from E. granulosus and expressed in Escherichia coli. Sequence analysis showed that EgP-29 contains 717-bp open reading frame and encodes a protein of 238 amino acid residues with a predicted molecular weight of 27.1 kDa. The recombinant EgP-29 (rEgP-29) could be recognized with anti-mice sera in Western blotting. The specific antibody was detected by enzyme-linked immunosorbent assay. Mice vaccinated with rEgP-29 and challenged intraperitoneally with E. granulosus protoscoleces revealed significant protective immunity of 96.6% (P < 0.05), compared with the control group. Thus, rEgP-29 protein is a promising candidate for an effective vaccine to prevent secondary echinococcosis.

Keywords    Echinococcus granulosus; diagnostic antigen P-29; cloning; expression; protective immunity

Received: July 30, 2008; Accepted: September 16, 2008

First Author: Zhiyun Shi. The Affiliated Hospital of Ningxia Medical University: Tel, 13895179732; E-mail, shizhiyun123@yahoo.com.cn


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