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Acta Biochimica et Biophysica Sinica 2009 41(1):63-68; doi:10.1093/abbs/gmn007
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© The Author 2009. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

PEA3 activates VEGF transcription in T47D and SKBR3 breast cancer cells

Dong Hua1,{dagger}, Bobin Chen2,{dagger}, Mei Bai1,{dagger}, Hao Yu1, Xiaohong Wu1 and Wei Jin1,3,*

1 Wuxi 4th People's Hospital, The 4th Affiliated Hospital of Suzhou University, Jiangsu Province, Wuxi 214062, China
2 Department of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, China
3 Department of Breast Surgery, Breast Cancer Institute, Cancer Hospital/Cancer Institute, Fudan University, Shanghai 200032, China

* Corresponding author: Tel, 86-13248179275; Fax, 86-510-85808820; E-mail, jinwei7207{at}hotmail.com


  Abstract

Vascular endothelial growth factor (VEGF) is a potent stimulator of angiogenesis and a prognostic factor for many tumors, including those of endocrine-responsive tissues such as the breast and uterus. In this study, we found that overexpression of PEA3 could increase VEGF mRNA levels and VEGF promoter activity in human T47D and SKBR3 breast cancer cells. Chromatin immunoprecipitation assay demonstrated that PEA3 could bind to the VEGF promoter in the cells transfected with PEA3 expression vector. PEA3 small interfering RNA attenuated VEGF promoter activity and the binding of PEA3 to the VEGF promoter in T47D and SKBR3 cells. These results indicated that PEA3 could activate VEGF promoter transcription.

Keywords    PEA3; VEGF promoter; transcription regulation

Received: June 28, 2008; Accepted: September 4, 2008

{dagger} These authors contributed equally to this study


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