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Acta Biochimica et Biophysica Sinica 2009 41(1):16-20; doi:10.1093/abbs/gmn002
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© The Author 2009. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

Protein engineering, expression, and activity of a novel fusion protein possessing keratinocyte growth factor 2 and fibronectin

Wonmo Kang and Junhyeog Jang*

Department of Biochemistry, Inha University School of Medicine, Incheon 400-712, Korea

* Corresponding author: Tel/Fax, 82-32-882-1877; E-mail, juhjang{at}inha.ac.kr


  Abstract

Growth factor-induced proliferation and differentiation often require adhesion of cells to the extracellular matrix proteins such as fibronectin (FN). In this study, we aimed to investigate the effect of protein engineering of the keratinocyte growth factor 2 (KGF2) fused to the FN on the mitogenic activity of KGF2. The fusion protein (KGF2-FN10), which was expressed in Escherichia coli, showed significantly enhanced mitogenic activity of KGF2 on human keratinocytes. Moreover, KGF2-FN10 fusion protein showed significantly increased activity to differentiate keratinocytes from native KGF2. In conclusion, these results suggest that KGF2-FN10 fusion protein has certain advantages over native KGF2 and may offer a novel strategy to potentiate the therapeutic effect of KGF2.

Keywords    fusion protein; growth factor; fibronectin; keratinocyte; keratinocyte growth factor 2

Received: June 17, 2008; Accepted: July 25, 2008
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